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- W2379662004 abstract "[Objective] To prepare polypeptide through the hydrolysis of Eupolyphaga sinensis protein with papain and then study the anticoagulant activity of the prepared polypeptide.[Method] The polypeptide was prepared with the method of enzymatic hydrolysis,and then the best preparation technology was determined according to the hydrolysis rate.The effects of different concentrations of polypeptide on the thrombin time(TT),activated partial thrombop lastin time(APTT) and prothrombin time(PT) in mice were measured with the kit.[Result] The best hydrolysis conditions were 8.0 pH,1.0% papain dose,55 ℃ hydrolysis temperature and 3.5 h hydrolysis time;the prepared polypeptide from E.sinensis significantly extended the TT and APTT in mice,but had no influence on PT.[Conclusion] The polypeptide prepared from E.sinensis under the best hydrolysis conditions is proved to have anticoagulant activity." @default.
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- W2379662004 date "2012-01-01" @default.
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- W2379662004 title "Preparation and Anticoagulant Activity of Polypeptide in Eupolyphaga sinensis Walker" @default.
- W2379662004 hasPublicationYear "2012" @default.
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