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- W2379664089 abstract "The patterns of hypermethylation in calcitonin(CT)gene of 58 cases with acute leukemia were detected by using polymerase chain reaction (PCR) in combination with HpaⅡ restriction endonuclease digestion .The DNA of the cells in the case group to be detected were digested by using HpaⅡ,which then were amplified by using two pairs of primers specific for the CT genes.The fragments of 566 bp and 1 4 kb were produced respectively.The positive rate of acute lymphoblastic leukemia was 71 4(25/35),and acute non lymphoblastic leukemia 78 2(18/23),the sensitivity being 3 logs.This study suggests that hypermethylation of the calcitonin gene 5′ flanking is the specific molcular marker for the leukemic cell colony." @default.
- W2379664089 created "2016-06-24" @default.
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- W2379664089 date "1997-01-01" @default.
- W2379664089 modified "2023-09-30" @default.
- W2379664089 title "Use of the HpaII PCR to detect hypermethylation of the calcitonin gene in acute leukemia" @default.
- W2379664089 hasPublicationYear "1997" @default.
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