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- W2379680952 abstract "Total RNA was extracted from small intestine of New Yangzhou laying hens at 200 day of age.RT-PCR was conducted subsequently based on published chicken Calbindin-D28k gene sequence(NM_205513.1).The expected amplified cDNA fragment was cloned into TA vector.Sequence analysis revealed that the cloned Calbindin-D28k gene,encoding 262 amino acids,was 789 bp in length which was 99.9% identity but only one nucleotide changed from G to T at site 760 bp compared with published sequence from GenBank database.The homology of Calbindin-D28k gene sequence between chicken and other species such as horse,human,mouse and frog was 79.3%、79.1%、77.4%、77.3%,respectively.Reconstruction plasmid of pCEP4-CaBP-D28k was carried out by inserting the fragment of Calbindin-D28k gene into eukaryotic expression vector pCEP4,and the target segment in recombinant plasmid was characterized and confirmed by restrictive endonuclease assay as well as sequence analysis.This research provide a novel means to study regulation mechanism of calcium metabolism in chickens through pCEP4-CaBP-D28k." @default.
- W2379680952 created "2016-06-24" @default.
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- W2379680952 date "2008-01-01" @default.
- W2379680952 modified "2023-09-25" @default.
- W2379680952 title "Gene Cloning,Sequencing and Eukaryotic Expression Plasmid Construction of Chicken Calbindin-D28k" @default.
- W2379680952 hasPublicationYear "2008" @default.
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