Matches in SemOpenAlex for { <https://semopenalex.org/work/W2379686326> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2379686326 abstract "Aim To explore the production of adrenomedullin (ADM), changes and significance of ADM, ADM receptor system-calcitonin receptor like receptor (CRLR) and receptor activity modifying proteins (RAMP) mRNA in calcified vascular smooth muscle cells (VSMC). Methods Calcification of cultured rat VSMC was produced by incubation with β-glycerophos phate. Content of ADM released by VSMC was measured by radioimmunoassay (RIA). The amount of ADM, CRLR and RMAP mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The intracellular calcium content, alkaline phosphatase activity and cellular 45 Ca2+ uptake were determined. Results The content of calcium, 45 Ca2+ uptake and alkaline phosphatase activity in calcified VSMC increased by 118% , 174% and 7-fold (all P 0.01), respectively, compared with control VSMC. Content of ADM in medium increased by 99% (P 0.01). Furthermore, it was found that the levels of ADM, CRLR, RAMP 2 and RAMP 3 mRNA in calcified cells were elevated by 78% , 93.7% , 91.8% and 109.5 % (all P 0.01), respectively, compared with control. The elevated levels of CRLR, RAMP 2 and RAMP 3 mRNA were positively correlated with ADM mRNA ( r = 0.83, 0.92 and 0.93, respectively, P 0.01) in calcified VSMC. Conclusion Calcified VSMC generated an increased amount of ADM, up-regulated gene expressions of ADM, CRLR, RAMP 2 and RAMP 3, which suggested that changes of autocrine/paracrine function of vessel might be involved in the regulation of vascular calcification ." @default.
- W2379686326 created "2016-06-24" @default.
- W2379686326 creator A5057131701 @default.
- W2379686326 date "2004-01-01" @default.
- W2379686326 modified "2023-10-05" @default.
- W2379686326 title "Upregulation of Gene Expression of Adrenomedullin and its Receptor System in Calcified Vascular Smooth Muscle Cells" @default.
- W2379686326 hasPublicationYear "2004" @default.
- W2379686326 type Work @default.
- W2379686326 sameAs 2379686326 @default.
- W2379686326 citedByCount "0" @default.
- W2379686326 crossrefType "journal-article" @default.
- W2379686326 hasAuthorship W2379686326A5057131701 @default.
- W2379686326 hasConcept C104317684 @default.
- W2379686326 hasConcept C105580179 @default.
- W2379686326 hasConcept C126322002 @default.
- W2379686326 hasConcept C127561419 @default.
- W2379686326 hasConcept C128240485 @default.
- W2379686326 hasConcept C134018914 @default.
- W2379686326 hasConcept C150194340 @default.
- W2379686326 hasConcept C160160445 @default.
- W2379686326 hasConcept C170493617 @default.
- W2379686326 hasConcept C176685330 @default.
- W2379686326 hasConcept C181199279 @default.
- W2379686326 hasConcept C185592680 @default.
- W2379686326 hasConcept C2777152744 @default.
- W2379686326 hasConcept C2779395532 @default.
- W2379686326 hasConcept C2780309369 @default.
- W2379686326 hasConcept C2992686903 @default.
- W2379686326 hasConcept C519063684 @default.
- W2379686326 hasConcept C55493867 @default.
- W2379686326 hasConcept C71924100 @default.
- W2379686326 hasConcept C7876069 @default.
- W2379686326 hasConcept C86803240 @default.
- W2379686326 hasConceptScore W2379686326C104317684 @default.
- W2379686326 hasConceptScore W2379686326C105580179 @default.
- W2379686326 hasConceptScore W2379686326C126322002 @default.
- W2379686326 hasConceptScore W2379686326C127561419 @default.
- W2379686326 hasConceptScore W2379686326C128240485 @default.
- W2379686326 hasConceptScore W2379686326C134018914 @default.
- W2379686326 hasConceptScore W2379686326C150194340 @default.
- W2379686326 hasConceptScore W2379686326C160160445 @default.
- W2379686326 hasConceptScore W2379686326C170493617 @default.
- W2379686326 hasConceptScore W2379686326C176685330 @default.
- W2379686326 hasConceptScore W2379686326C181199279 @default.
- W2379686326 hasConceptScore W2379686326C185592680 @default.
- W2379686326 hasConceptScore W2379686326C2777152744 @default.
- W2379686326 hasConceptScore W2379686326C2779395532 @default.
- W2379686326 hasConceptScore W2379686326C2780309369 @default.
- W2379686326 hasConceptScore W2379686326C2992686903 @default.
- W2379686326 hasConceptScore W2379686326C519063684 @default.
- W2379686326 hasConceptScore W2379686326C55493867 @default.
- W2379686326 hasConceptScore W2379686326C71924100 @default.
- W2379686326 hasConceptScore W2379686326C7876069 @default.
- W2379686326 hasConceptScore W2379686326C86803240 @default.
- W2379686326 hasOpenAccess W2379686326 @default.
- W2379686326 hasRelatedWork W1999069103 @default.
- W2379686326 hasRelatedWork W2000748391 @default.
- W2379686326 hasRelatedWork W2023541036 @default.
- W2379686326 hasRelatedWork W2159675189 @default.
- W2379686326 hasRelatedWork W2353775091 @default.
- W2379686326 hasRelatedWork W2355611137 @default.
- W2379686326 hasRelatedWork W2357052066 @default.
- W2379686326 hasRelatedWork W2359147752 @default.
- W2379686326 hasRelatedWork W2360314184 @default.
- W2379686326 hasRelatedWork W2360864781 @default.
- W2379686326 hasRelatedWork W2361616459 @default.
- W2379686326 hasRelatedWork W2368557176 @default.
- W2379686326 hasRelatedWork W2379397368 @default.
- W2379686326 hasRelatedWork W2383371172 @default.
- W2379686326 hasRelatedWork W2384141348 @default.
- W2379686326 hasRelatedWork W2384490165 @default.
- W2379686326 hasRelatedWork W2384690458 @default.
- W2379686326 hasRelatedWork W2385882682 @default.
- W2379686326 hasRelatedWork W2389546790 @default.
- W2379686326 hasRelatedWork W3162727892 @default.
- W2379686326 isParatext "false" @default.
- W2379686326 isRetracted "false" @default.
- W2379686326 magId "2379686326" @default.
- W2379686326 workType "article" @default.