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- W2379715495 abstract "Objective: Test the influence of trans-IGF-1 gene on cell proliferation in vitro. Methods: Firstly a DNA fragment coding both signal and mature peptide of IGF-1 was subcloned into reporter gene plasmid pTracer-CMV/Bsd. Therefore recombinant plasmid pTracer-cIGF-1 was delivered into C2C12 mouse myoblast celline by lipofectamine 2000. Screened steadily transfected cells by selected media containing blasticidin 5 μg/mL, and 3H-TdR method was applied to detect proliferation rate of the cells. Results: 48 h after transfection green areas similar with the shape of cell could be seen under fluroscent microscope. As a result, trans-IGF-1 cell had an more increased proliferation rate than untransfected C2C12 cell and the media of trans-IGF-1 cell could also accelerated the proliferation rate of cultured C2C12 cell markedly. Conclusion: Transfected IGF-1 gene could by expressed steadily in host cell. IGF-1 synthesized by the trans-IGF-1 cell could be secreted out of the cell and could enhance cell proliferation locally." @default.
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- W2379715495 date "2004-01-01" @default.
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- W2379715495 title "The Influence of Trans-IGF-1 Gene on Cell Proliferation" @default.
- W2379715495 hasPublicationYear "2004" @default.
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