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- W2379717650 abstract "Objective To explore an optimal method for hypothermic preservation of hepatocytes at 4°C. Methods Primary porcine hepatocytes were harvested by modified two step collagenase perfusion method, then hypothermically preserved for 24h、48h or 72h before plated under culture medium conditions. The cells were stored either in the RPMI 1640 medium with or without polyethylene glycol, or in the UW(University of Wisconsin solution), the most effective solution for cold preservation of liver. After cold preservation, their viability, attachment rate and drug metabolic function were measured, and the ultrastructural changes were observed through light and electron microscopy. Results After 24h、48h or 72h of storage at 4°C in RPMI 1640 medium, cell viability and adherence rate were significant reduced, but the hepatocytes maintained in PEG or in the UW kept their function well, and their total protein secretion, extracellular LDH and drug metabolic reaction were similar to those found with fresh hepatocytes. Conclusion Primary porcine hepatocytes can be preserved for 48 hours. Addition of polyethylene glycol to the RPMI 1640 medium results in slightly greater viability and better function of hepatocytes after cold presearvation." @default.
- W2379717650 created "2016-06-24" @default.
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- W2379717650 date "2002-01-01" @default.
- W2379717650 modified "2023-09-28" @default.
- W2379717650 title "Hypothermic preservation of primary porcine hepatocytes" @default.
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