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- W2379727405 abstract "To construct the human umbilical card mesenehymal stem cells(hUCMSCs)infected with the recombinant lentivirus containing IL-21 gene,and to provide a new method for targeted treatment of cancer,the fragment of IL-21 gene from vector pRSC-IL-21 was cloned into the lentivirus vector pHAGE-CMV-MCS-IzsGreen and the lentivirus was packaged and subsequently transfected into the human embryo kidney HEK 293 T cells.The expression of IL-21 was detected in the recombinant lentivirus infected hUCMSCs by Western blot analysis.The results showed that the lentivirus vector carrying the IL-21 gene was established successfully.The expression of green fluorescent protein was observed under a fluorescence microscope after the recombinant lentivirus was cotransfected into HEK 293 T cells.The expression of IL-21 was detected from the recombinant lentivirus infected hUCMSCs and the expressed IL-21 in the supernatant could induce spleen cell proliferation.These results indicate that the correctly constructed recombinant lentivirus containing IL-21 gene could infect the hUCMSCs that express functional IL-21." @default.
- W2379727405 created "2016-06-24" @default.
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- W2379727405 date "2014-01-01" @default.
- W2379727405 modified "2023-09-23" @default.
- W2379727405 title "Identification of human umbilical card mesenchymal stem cells infected with lentivirus vector encoding IL-21 and preliminary investigation into the functional activity of expressed IL-21" @default.
- W2379727405 hasPublicationYear "2014" @default.
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