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- W2379781446 abstract "The study aimed to set up the ISSR reaction system of the flowering Chinese Cabbage,including the genomic DNA extraction,amplification,cycle time,primers screening.The results showed that the optimum temperature of a eggplant amplification was 48.0℃,and the best cycle time was 45cycle.4 primers were screened out from 49 ISSR primers totally to amplify the flowering Chinese Cabbage genome DNAs of 6 materials effectively,and 32 sites were amplified out totally,including 22 polymorphism sites with polymorphism rate of 68.80%.All of the 6 varieties could be differentiated from each other based on the fingerprints established by these specific bands.The Jaccard genetic similarity coefficients among flowering Chinese Cabbage vrieties were calculated by NTSYS-pc2.10e software.The genetic similarity coefficients among 6 flowering Chinese Cabbage varieties were between 0.19 and 0.77.The ISSR Markers showed that the higher genetic diversity of between materials.This study provided theoretical foundation to the seed purity identification and new varieties breeding of the chinese cabbage." @default.
- W2379781446 created "2016-06-24" @default.
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- W2379781446 date "2010-01-01" @default.
- W2379781446 modified "2023-09-24" @default.
- W2379781446 title "Preliminary Study on Identification Flowering Chinese Cabbage Varieties by ISSR Molecular Markers" @default.
- W2379781446 hasPublicationYear "2010" @default.
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