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- W2379808781 abstract "[Objective] To investigate the methods of isolated, culture, differentiation and ultrastructure of the neural stem cells(NSCs). [Methods] The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, subventricular zone(SVZ), etc. Immunofluorescence was applied to identify the neural stem cells and their potential of differentiation. At the same time ultrastructure of the NSCs was observed by electronic microscopy. [Results] The cell clones isolated from embryonic rat cortex, subventricular zone possess the ability of proliferation, self-reproduction. neurospheres with microvilli -like cytoplasmic processes protruding from its surface, the conjuction between inner cells in it was loose, apoptotic bodies in it was observed. Some cells differentiated cells having axon structure. [Conclusion] The cultured neural stem cells possess the ability of proliferation , self-reproduction and multi-differential potential and correspondent ultrastructure with function." @default.
- W2379808781 created "2016-06-24" @default.
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- W2379808781 date "2006-01-01" @default.
- W2379808781 modified "2023-09-25" @default.
- W2379808781 title "Study on isolated culture and ultrastructure of neural stem cells" @default.
- W2379808781 hasPublicationYear "2006" @default.
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