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- W2379849969 abstract "To establish a stable and convenient method for preparation of highly purified trophoblast cells from sheep placenta and to provide the cellular basis for the study of cell transfection and RNA interference test,the sheep trophoblast cells were cultured by explant culture technique,and purified by digestion differential method.Phase-contrast microscope was used to observe the morphological characteristics of trophoblast cells,and general HE staining,immunohistochemical staining,electron microscopy and RT-PCR technology were used to identify the cell source.Inverted phase contrast microscope observation showed that the cells were the epithelial-like cell morphology,paving-like growth.Keratin staining showed that the purity of the cultured cells exceeded 90%,vimentin staining was negative.The unique structure of trophoblast cells could be observed by transmission electron microscopy.The cell viability evaluated by trypan blue exclusion test was greater than 95%.Conventional RT-PCR technique can detect trophoblast cells secreted interferon specific protein-1 gene.The results showed that this primary culture method can provide highly purified sheep trophoblast cells with good cellular viability.The present study provides an experimental basis for our laboratory in vitro studies." @default.
- W2379849969 created "2016-06-24" @default.
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- W2379849969 date "2012-01-01" @default.
- W2379849969 modified "2023-09-27" @default.
- W2379849969 title "Culture and identification of sheep trophoblast cells in vitro" @default.
- W2379849969 hasPublicationYear "2012" @default.
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