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- W2379862478 abstract "Objective To assess the effect of detecting the specific antibody and the SARS-cov gene of SARS patients. Methods The specific IgG and IgM antibody in the serum of the SARS patients in different period , the healthy and feverous patients without SARS were detected by ELISA ;the SARS-cov RNA in the sputum of these groups’ were detected by nested RT-PCR. Results During 6 days～57 days of hospitalization , the SARS-cov gene in the sputum of 56 SARS patients were detected by nested RT-PCR, 34 of 56 cases are positive, and the positive rate is 60.7 %; the specific antibodies in 32 of 56 cases are positive by indirect ELISA, and the positive rate is 57.1 %.15 Cases are positive and 6 cases are negative by two pathogen diagnostic methods. 20 Cases of the healthy and 15 cases of general fever patients are all negative by two pathogen diagnostic methods. during 6 days～13 days of hospitalization, the positive rate of SARS-cov RNA in the sputum of SARS by the nested RT-PCR(92.3 %) is markedly higher than that of the specific antibodies in the serum of the SARS patients by the indirect ELISA(7.7 %); during 35days of hospitalization, the positive rate of SARS-cov RNA in the sputum of SARS by the nested RT-PCR(36.8 %) is markedly lower than that of the specific antibodies in the serum of the SARS patients by the indirect ELISA(94.7 %)(P0.01).Conclusion The early SARS patients could be diagnosed by detecting the SARS-cov gene in the sputum of SARS patients. It is of practical significance for diagnosing and curing the SARS patients and excluding the suspicious cases early. It is of limited sense for diagnosis and identification of SARS to detect the specific antibody in the serum of SARS patients." @default.
- W2379862478 created "2016-06-24" @default.
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- W2379862478 date "2004-01-01" @default.
- W2379862478 modified "2023-09-23" @default.
- W2379862478 title "An Evaluation on Detecting the Specific Antibody and SARS-cov Gene of SARS Patients" @default.
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