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- W2379888016 abstract "Objective: To use a technique of rapid and automatical with high through-put for deletional α-thalassemia gene in Jiangxi provice.Methods:Using The Real-Time PCR and dissociation curve analysis(D.C) and mPCR method to detect 3 deletional α-thalassemia gene,namely-α3.7,-α4.2(single gene deletion),and——SEA(South East Asia double gene deletion).Results:Sensitivity of the SYBR-Q-PCR-based method was at least 16 times higher than the mPCR/gel electrophoresis method.Using The Real-Time PCR combined with the DC analysis technique and using mPCR method to detect 3 deletional α-thalassemia gene,In 19cases of deletional α-Thal,including-SEA/-α4.2-SEA/-α3.7-α3.7/-α3.7-α4.2/-α4.2-α3.7/-α4.2.Conclusion: SYBR-Q-PCRand DC were shown to be the same as mPCR is simples,time-saving,economic and accurate method for detecting common deletional α-thalassemia gene.The Real-Time PCR combined with the DC analysis technique is a sensitivity,rapid and automatical with high through –put and without dual fluorescent labeled probe." @default.
- W2379888016 created "2016-06-24" @default.
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- W2379888016 date "2009-01-01" @default.
- W2379888016 modified "2023-09-23" @default.
- W2379888016 title "Detection of three common deletional α-thalassemia gene in Jiangxi provice by using the real-time PCR combined with the dissociation curve analysis and mPCR" @default.
- W2379888016 hasPublicationYear "2009" @default.
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