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- W2379900377 abstract "An isolate of Tobacco etch virus (TEV) was obtained from tobacco plants in Shandong Province, which was named at TEV-SD1. The coat protein (CP) gene of TEV-SD1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR), and was subcloned into pET-22b(+) prokaryotic expression vector. The recombinant vector pETEV-CP was transformed into BL21 host strain of E.coli. Sequence analysis revealed that the CP genes was 789 nucleotides in length, which encodes the coat protein of 263 amino acids, and the CP genes shared 94.1%-98.6% nucleotides and amino acid homology with TEV CP genes of five strains registered in GenBank. The target fusion peptide with molecular weight of 33kDa was expressed under the condition of 37℃ and induced by IPTG at the final concentration of 1 mmol/L. Rabbit was immunized using the expressed target peptide as antigen, and the antiserum was obtained. The antiserum gave a titer of 1/2048 with high specificity to TEV." @default.
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- W2379900377 date "2005-01-01" @default.
- W2379900377 modified "2023-09-23" @default.
- W2379900377 title "Cloning and prokaryotic expression of coat protein gene of {sl Tobacco etch virus} and preparation of viral-specific antiserum" @default.
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