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- W2379934200 abstract "Objective:To explore the role of p53 in ceramide-induced endothelial cells apoptosis. Method:Human umbilical vein endothelial cells(HUVEC) were treated with different dose of C2-ceramide;And endothelial cells were primarily cultured and treated with C2-ceramide alone or in combination with PFT-α(an inhibitor of p53) respectively. The apoptosis of cells was detected with Tunel. The expression of p53 protein and mRNA levels was measured by Western blot and reverse transcription polymerase chain reaction (RT-PCR). Result:C2-ceramide induces HUVEC apoptosis in a dose-dependent manner. Ceramide decreased the expressions of p53. PFT-α can not inhibit the HUVEC apoptosis induced by C2-ceramide. Conclusion:Ceramide-induced endothelial cells apoptosis is independent on p53-independent pathway." @default.
- W2379934200 created "2016-06-24" @default.
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- W2379934200 date "2008-01-01" @default.
- W2379934200 modified "2023-09-28" @default.
- W2379934200 title "Study on the role of p53 in ceramide-induced endothelial cells apoptosis" @default.
- W2379934200 hasPublicationYear "2008" @default.
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