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- W2379936601 abstract "Objective To investigate the method and feasibility of transfection on SOX-9 gene into marrow stroma cell(BMSCs)of rabbits.Methods The eukaryotic expression vectors that were constructed by SOX-9 cDNA were transfected into BMSCs of rabbits by liposome induction.The transcription and expression of SOX-9 gene in BMSCs were detected by morphology observation mean,immunohistochemistry,enzyme linked immunosorbent assay and reverse transcription-polymerasechain reaction(RT-PCR)means.The changes of biologic characterisctics of transfected BMSCs were also observed.Results The stable expression of SOX-9 proteins was detected in the experimental group by immunohistochemistry,and it was higher in 2 weeks than in 6 days.The expression of SOX-9 gene were observed only in experimental group by RT-PCR means.The expression of SOX-9 protein were detected in three group by ELISA means,and it was remarkably higher in the experimental group than other group(P0.01),and it was the highest at 48h in the experimental group.Conclusion The BMSCs of rabbits can be transfected and are induced to differentiate towards cartilage by SOX-9 gene." @default.
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- W2379936601 date "2008-01-01" @default.
- W2379936601 modified "2023-09-24" @default.
- W2379936601 title "Experimental study on the bone marrow stromal cells of rabbits transfected by SOX-9 gene" @default.
- W2379936601 hasPublicationYear "2008" @default.
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