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- W2379964369 abstract "Objective To study the selective killing effect of adenoviru(Ads)-mediated double suicide gene(CD/TK)under the regulation of the human vascular endothelial growth factor(VEGF)promoter on breast cancer cells MCF-7.Methods The VEGF-expressing MCF-7 cells and non VEGF-expressing normal human mammary epithelial cells in primary culture were infected by the Ad-VEGFP-CD/TK.The infection efficiencies were observed under a fluorescence microscope.Followed by treatment with GCV and 5-FC,the killing effects were evaluated and bystander effects were analyzed by MTT assay.Distribution of cell cycle and DNA content in MCF-7 were detected by flow cytometry.An animal model of human breast cancer in nude mice was reproduced.Ad-VEGFP-CD/TK was injected directly into the tumor.Subsequently,the nude mice were given intraperitoneal injection of GCV[50 mg/(kg·d)] and 5-FC[500 mg/(kg·d)] daily for 14 days.The inhibition rates for tumor growth were also calculated.Results The infection rates of the resultant recombinant Ads to MCF-7 and human mammary epithelial cells were not significantly different,and the rates increased gradually with the addition of multiple of infection(MOI)of Ads.MTT assay showed:MCF-7 cells infected with Ads were highly sensitive to the pro-drugs,while the infected human mammary epithelial cells were not sensitive.There was considerable bystander effect in MCF-7 cells.At the multiplicity of infection(MOI)of 100,apoptotic peak was also shown in pro-drug group by flow cytometry.The analysis of cell cycle revealed that the rate of cells at the G0-G1 phase increased and the rate at the G2-M and S phase decreased by treatment with pro-drug using flow cytometry.Tumors size decreased during treatment of double suicide gene system by establish subcutaneous transplant model.Conclusion The CD/TK fusion gene system controlled by VEGF promoter has selective killing effects on the VEGF-expressing MCF-7 cells and induces cell apoptosis.It significantly inhibits the growth of xenografted breast cancer in nude mice." @default.
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- W2379964369 date "2008-01-01" @default.
- W2379964369 modified "2023-09-26" @default.
- W2379964369 title "VEGF-mediated double suicide gene selectively kills breast cancer cells MCF-7" @default.
- W2379964369 hasPublicationYear "2008" @default.
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