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- W2379970250 abstract "GhcelA1 cDNA full length which reported in GeneBank(U58283) was cloned from Coker 312(Gossypium hirsutum) fiber at 20 DPA(days after anthesis) for the first time using RT-PCR method.A serial of plant expression vectors for over-expression,fusion gene with EGFP(Enhanced Green Fluorescent Protein) and RNAi(interference) by recombination technology were constructed and confirmed by molecular and sequence methods.Expression of EGFP in infected cells was clearly evident in confocal microscopy(Zeiss LSM-510) experiments.This showed that the GhcelA1-EGFP fusion in correct construction and could express in cotton cells successfully.Clone and construction were the precondition of transformation for culture improved variety and studying on the pivotal function of cellulose synthetase in the second cell wall thicken period of cotton fiber." @default.
- W2379970250 created "2016-06-24" @default.
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- W2379970250 date "2009-01-01" @default.
- W2379970250 modified "2023-09-28" @default.
- W2379970250 title "Cloning and Construction of GhcelA1 Gene in Cotton Fiber" @default.
- W2379970250 hasPublicationYear "2009" @default.
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