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- W2379973317 abstract "The hypocotyl of Brassica napus L.Huashuang No.4 was transformed mediated by Agrobacterum tumefaciens and the expression of the reporter gene gus was detected by histochemical pigmentation.The effects of Agrobacterium culture method and hormone combination in the pre-culture medium on transformation!were analyzed.The results showed that subculture of Agrobacterium in MS with AS for some time after activating in LB medium promoted transformation.Obvious differences were observed among the six Agrobacterium culture methods adopted,of which s2-5 gave the best transformation result.Combination of 2,4-D with 6-BA in pre-culture had better effect than others.The combination of 2.00 mg/L 2,4-D and 0.25 mg/L 6BA was the best in transformation efficiency.The experiments also showed that resistant callus percentage was not necessarily correlated with transformation efficiency." @default.
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- W2379973317 date "2007-01-01" @default.
- W2379973317 modified "2023-09-24" @default.
- W2379973317 title "Effects of Agrobacterium Culture Methods and Hormone Combinations in Pre-culture Medium on Hypocotyle Transformation in Brassica napus" @default.
- W2379973317 hasPublicationYear "2007" @default.
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