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• W2379999130 abstract "Objective To investigate the mechanism of macrophage receptor with collagenous structure( MARCO) induced alveolar macrophage(AM) mitochondrial apoptosis and the role of which in the generation and development of silicosis. Methods Forty-eight specific pathogen free adult male SD rats weighted 180-220 g were randomly divided into 3 groups,a polyguanylic acid(PolyG) group,a physiological saline group and a silicosis model group with 16 rats in each group. Silicosis model group and PolyG group were intratracheally given silica(50. 0 g / L) 1. 0 ml to establish the silicosis model,the physiological saline group was injected with the same dose of physiological saline in parallel,PolyG group was treated with PolyG via tail vein one time while others were treated with the same dose of physiological saline from the right day of establishing silicosis model,all animals could eat and drink ad libitum. Animals were sacrificed 28 days and 56 days after exposure with 8 rats in each group in a time,the left side lung lavage was collected,AMs were separated and purified. The other side of the lung tissue was fixed with paraformaldehyde and stained by hematoxylin-eosin( HE),the pathological change and the severity of pulmonary fibrosis was observed under light microscope. The contents of MARCO, B cell lymphoma / lewkmia-2(Bcl-2),Bcl-2 associated X protein(Bax),cytochrome c(CytC),cysteinyl aspartate specific protease-9(caspase-9) and caspase-3 were detected by western blotting. mRNA expression levels of type Ⅰ and type Ⅲ procollagens were measured by real-time PCR. Reactive oxygen species(ROS) contents and mitochondrial transmembrane potential changes of AMs were detected by flow cytometry. Results MARCO,Bax,CytC,Caspase-9,Caspase-3 contents,type Ⅰ and type Ⅲ procollagen mRNA relative expression levels,AM ROS positive rate and mitochondrial depolarization rate in the saline group and PolyG group in day 28 and 56 were lower than those in the silicosis model group(P 0. 01) at the same period,but Bcl-2 was higher(P 0. 01). No statistical significances were found between the saline and the PolyG group on the indexes of relative expression level of MARCO,CytC and Caspase-3( P 0. 05) at day 28,and no statistical significances were observed between the saline and the PolyG group on the indexes of relative expression level of MARCO,CytC and Caspase-3 and Bax(P 0. 05) at day 56. Compared with the above 10 indexes at day 28 in the saline group,all variables at day 56 were statistically unchanged(P 0. 05) in the saline group. Compared with the data at day 28 in the silicosis model group,AM ROS positive rate and mitochondrial depolarization rate remained unchanged(P 0. 05),Bcl-2 relative expression level decreased(P 0. 01) while other 7 variables increased(P 0. 01) at day 56. Compared with the data at day 28 in the PolyG group,CytC,Bax relative expression levels,AM ROS positive rate and mitochondrial depolarization rate decreased(P 0. 01) at day 56 while other variables showed no statistical significance(P 0. 05). Conclusion MARCO may play an important role in AM mitochondrial apoptosis pathway." @default.
• W2379999130 created "2016-06-24" @default.
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• W2379999130 date "2014-01-01" @default.
• W2379999130 modified "2023-09-27" @default.
• W2379999130 title "Study on MARCO induced mitochondrial apoptosis pathway in alveolar macrophage in rats exposed to silica dust" @default.
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