Matches in SemOpenAlex for { <https://semopenalex.org/work/W2380025394> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2380025394 abstract "Objective Subcutaneous adipose-derived cell populations would be isolated and cultured in vitro.In accordance with international standard,we will confirm that cell populations obtained in vitro are adipose-derived mesenchymal stem cells by morphology,immunophenotyping and identifying of multiple differentiation potential.Methods Using dualdigestion method for separation of cells in vitro with 0.1% collagenase I and 0.25% trypsin to get it,adding LG-DMEM medium containing the volume fraction of 10% fetal bovine serum,culturing at 37℃ and 5% of CO 2 saturated humidity conditions,then amplifying of digestion and passage until the cell growth to 70% to 80%.Cell morphology would be observed through inverted microscope.Flow cytometry detect cell phenotype expression.The third passage cells would be directed induction of differentiation by added osteogenic and adipogenic induction agents into the culture medium,and then induced cells would be staining.Results Isloated cell populations are long fusiform and whirlpool-like adherent growth.P2 cells detected by Flow cytometry,which of CD73 and CD105,C90,CD44,CD49d and CD29 are positive expression,but of CD14 and CD34,CD45,CD106and HLA-DR are negative expression.Alizarin red staining,alkaline phosphatase staining and VON-KOSSA stain tested positive 3 weeks after osteogenic induction.2 weeks after the adipogenic induction,there are small lipid droplets appearing,and oil red O staining is positive.Conclusion The Cells that using collagenase and trypsin digestion method isolated from adipose tissue can be attached to the wall growth.Flow cytometry confirme that isolated cells can express the mesenchyme stem cells consistent phenotype and have osteogenic and adipogenic differentiation ability after directed induced,the cultured cells are consistent with the international standard about the characteristics of mesenchyme stem cells." @default.
- W2380025394 created "2016-06-24" @default.
- W2380025394 creator A5027725869 @default.
- W2380025394 date "2013-01-01" @default.
- W2380025394 modified "2023-09-24" @default.
- W2380025394 title "Identification of cultured human adipose-derived stem cells in vitro" @default.
- W2380025394 hasPublicationYear "2013" @default.
- W2380025394 type Work @default.
- W2380025394 sameAs 2380025394 @default.
- W2380025394 citedByCount "0" @default.
- W2380025394 crossrefType "journal-article" @default.
- W2380025394 hasAuthorship W2380025394A5027725869 @default.
- W2380025394 hasConcept C10205521 @default.
- W2380025394 hasConcept C116716535 @default.
- W2380025394 hasConcept C122927707 @default.
- W2380025394 hasConcept C153911025 @default.
- W2380025394 hasConcept C160160445 @default.
- W2380025394 hasConcept C171089720 @default.
- W2380025394 hasConcept C181199279 @default.
- W2380025394 hasConcept C185592680 @default.
- W2380025394 hasConcept C198826908 @default.
- W2380025394 hasConcept C202751555 @default.
- W2380025394 hasConcept C2778394429 @default.
- W2380025394 hasConcept C2779404806 @default.
- W2380025394 hasConcept C2780806160 @default.
- W2380025394 hasConcept C2780932548 @default.
- W2380025394 hasConcept C28328180 @default.
- W2380025394 hasConcept C54355233 @default.
- W2380025394 hasConcept C553184892 @default.
- W2380025394 hasConcept C55493867 @default.
- W2380025394 hasConcept C74864618 @default.
- W2380025394 hasConcept C86803240 @default.
- W2380025394 hasConcept C95444343 @default.
- W2380025394 hasConceptScore W2380025394C10205521 @default.
- W2380025394 hasConceptScore W2380025394C116716535 @default.
- W2380025394 hasConceptScore W2380025394C122927707 @default.
- W2380025394 hasConceptScore W2380025394C153911025 @default.
- W2380025394 hasConceptScore W2380025394C160160445 @default.
- W2380025394 hasConceptScore W2380025394C171089720 @default.
- W2380025394 hasConceptScore W2380025394C181199279 @default.
- W2380025394 hasConceptScore W2380025394C185592680 @default.
- W2380025394 hasConceptScore W2380025394C198826908 @default.
- W2380025394 hasConceptScore W2380025394C202751555 @default.
- W2380025394 hasConceptScore W2380025394C2778394429 @default.
- W2380025394 hasConceptScore W2380025394C2779404806 @default.
- W2380025394 hasConceptScore W2380025394C2780806160 @default.
- W2380025394 hasConceptScore W2380025394C2780932548 @default.
- W2380025394 hasConceptScore W2380025394C28328180 @default.
- W2380025394 hasConceptScore W2380025394C54355233 @default.
- W2380025394 hasConceptScore W2380025394C553184892 @default.
- W2380025394 hasConceptScore W2380025394C55493867 @default.
- W2380025394 hasConceptScore W2380025394C74864618 @default.
- W2380025394 hasConceptScore W2380025394C86803240 @default.
- W2380025394 hasConceptScore W2380025394C95444343 @default.
- W2380025394 hasLocation W23800253941 @default.
- W2380025394 hasOpenAccess W2380025394 @default.
- W2380025394 hasPrimaryLocation W23800253941 @default.
- W2380025394 hasRelatedWork W2060599105 @default.
- W2380025394 hasRelatedWork W2182939160 @default.
- W2380025394 hasRelatedWork W2351099705 @default.
- W2380025394 hasRelatedWork W2360342517 @default.
- W2380025394 hasRelatedWork W2361190613 @default.
- W2380025394 hasRelatedWork W2362286314 @default.
- W2380025394 hasRelatedWork W2366020751 @default.
- W2380025394 hasRelatedWork W2373499966 @default.
- W2380025394 hasRelatedWork W2375017907 @default.
- W2380025394 hasRelatedWork W2377592276 @default.
- W2380025394 hasRelatedWork W2377839254 @default.
- W2380025394 hasRelatedWork W2382247682 @default.
- W2380025394 hasRelatedWork W2393149010 @default.
- W2380025394 hasRelatedWork W2419123116 @default.
- W2380025394 hasRelatedWork W3029423528 @default.
- W2380025394 hasRelatedWork W3031215290 @default.
- W2380025394 hasRelatedWork W3031691698 @default.
- W2380025394 hasRelatedWork W3031832314 @default.
- W2380025394 hasRelatedWork W3147830939 @default.
- W2380025394 hasRelatedWork W85771254 @default.
- W2380025394 isParatext "false" @default.
- W2380025394 isRetracted "false" @default.
- W2380025394 magId "2380025394" @default.
- W2380025394 workType "article" @default.