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- W2380032425 abstract "Objective To provide a PCR-RFLP method for unequivocal differentiation of C.dubliniensis from C.albicans.Methods All 41 clinical isolates were sampled from patients in the dermatology department of Tianjin General Hospital.Standardisolates of C.albicans(ATCC-11006)and C.dublinniensis(CBS-8501)were presented by Peking University.All clinical isolates were identified using phenotypic tests and grew poorly at 45℃ on SDA.The PCR primersⅠand Ⅱ binded to conserved regions of the Internal Transcribed Spacer regions ITS1 or ITS2,respectively.PCR products were digested for 14h at 37℃ with HpyF10VI.Digestion were run on 8% PAGE.Results Thirty-nine clinical isolates were confirmed to be C.albicans,which showed the same RFLP pattern as standard isolates of C.albicans.The other 2 clinical isolates show different RFLP pattens.After sequencing,one was confirmed to be C.albicans,the other maybe was C.dublinniensis.Conclusion Our PCR-RFLP method for two Candida species discrimination have been established.Sequencing is a helpful supplement." @default.
- W2380032425 created "2016-06-24" @default.
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- W2380032425 date "2008-01-01" @default.
- W2380032425 modified "2023-09-26" @default.
- W2380032425 title "Identification of Candida dubliniensis with PCR-RFLP analysis" @default.
- W2380032425 hasPublicationYear "2008" @default.
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