Matches in SemOpenAlex for { <https://semopenalex.org/work/W2380039300> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2380039300 abstract "Objective To investigate the effects of tumor necrosis factor α (TNF α) on β adrenoceptor (β AR) and β AR related G protein coupled receptor kinases (GRKs) in rat pulmonary microvascular endothelial cells (PMVECs) as well as the interfering action of anisodamine. Methods Radio ligand binding assay was used to measure the maximal binding capacity (B max ) changes of β AR in rat PMVECs after treatment with TNF α. The effects of TNF α and β AR related GRKs expression at the protein level were observed by Western blotting. Results B max of β AR in normal rat PMVECs was (5.58±0.31) fmol/10 5 cells. B max of β AR in TNF α group decreased significantly as compared with that in the normal control group, but no significant difference was found between the normal control group and TNF α+anisodamine group. The expression of GRK2 in rat PMVECs was positive, but expression of GRK3, GRK5, and GRK6 were negative. The expression of GRK2 in TNF α group and TNF α+anisodamine group increased significantly as compared with that in the normal control group, but no significant difference was found between the TNF α group and the TNF α+anisodamine group. Conclusion GRK2 but not GRK3, GRK5, or GRK6 is expressed in rat PMVECs. The increased expression of GRK2 induced by TNF α in rat PMVECs might promote the phosphorylation of β AR, leading to β AR internalization and decoupling with G protein, which might be the main mechanism of down regulation of β AR induced by TNF α. Anisodamine might inhibit the down regulation of β AR through other mechanism instead of inhibiting the increase of GRK2 expression." @default.
- W2380039300 created "2016-06-24" @default.
- W2380039300 creator A5013607405 @default.
- W2380039300 date "2004-01-01" @default.
- W2380039300 modified "2023-09-23" @default.
- W2380039300 title "Effects of tumor necrosis factor alpha on beta-adrenoceptor and beta-adrenoceptor related GRKs in rat PMVEC" @default.
- W2380039300 hasPublicationYear "2004" @default.
- W2380039300 type Work @default.
- W2380039300 sameAs 2380039300 @default.
- W2380039300 citedByCount "0" @default.
- W2380039300 crossrefType "journal-article" @default.
- W2380039300 hasAuthorship W2380039300A5013607405 @default.
- W2380039300 hasConcept C104317684 @default.
- W2380039300 hasConcept C126322002 @default.
- W2380039300 hasConcept C134018914 @default.
- W2380039300 hasConcept C135285700 @default.
- W2380039300 hasConcept C170493617 @default.
- W2380039300 hasConcept C17991360 @default.
- W2380039300 hasConcept C185592680 @default.
- W2380039300 hasConcept C2779686630 @default.
- W2380039300 hasConcept C33235085 @default.
- W2380039300 hasConcept C55493867 @default.
- W2380039300 hasConcept C71924100 @default.
- W2380039300 hasConcept C78976303 @default.
- W2380039300 hasConcept C80631254 @default.
- W2380039300 hasConcept C85265743 @default.
- W2380039300 hasConcept C86803240 @default.
- W2380039300 hasConceptScore W2380039300C104317684 @default.
- W2380039300 hasConceptScore W2380039300C126322002 @default.
- W2380039300 hasConceptScore W2380039300C134018914 @default.
- W2380039300 hasConceptScore W2380039300C135285700 @default.
- W2380039300 hasConceptScore W2380039300C170493617 @default.
- W2380039300 hasConceptScore W2380039300C17991360 @default.
- W2380039300 hasConceptScore W2380039300C185592680 @default.
- W2380039300 hasConceptScore W2380039300C2779686630 @default.
- W2380039300 hasConceptScore W2380039300C33235085 @default.
- W2380039300 hasConceptScore W2380039300C55493867 @default.
- W2380039300 hasConceptScore W2380039300C71924100 @default.
- W2380039300 hasConceptScore W2380039300C78976303 @default.
- W2380039300 hasConceptScore W2380039300C80631254 @default.
- W2380039300 hasConceptScore W2380039300C85265743 @default.
- W2380039300 hasConceptScore W2380039300C86803240 @default.
- W2380039300 hasOpenAccess W2380039300 @default.
- W2380039300 hasRelatedWork W1561993052 @default.
- W2380039300 hasRelatedWork W185382499 @default.
- W2380039300 hasRelatedWork W1973723129 @default.
- W2380039300 hasRelatedWork W2078775750 @default.
- W2380039300 hasRelatedWork W2112184650 @default.
- W2380039300 hasRelatedWork W2113860061 @default.
- W2380039300 hasRelatedWork W2126970484 @default.
- W2380039300 hasRelatedWork W2141559626 @default.
- W2380039300 hasRelatedWork W2155665730 @default.
- W2380039300 hasRelatedWork W2181530308 @default.
- W2380039300 hasRelatedWork W2283779510 @default.
- W2380039300 hasRelatedWork W2349814773 @default.
- W2380039300 hasRelatedWork W2351261391 @default.
- W2380039300 hasRelatedWork W2360803201 @default.
- W2380039300 hasRelatedWork W2378234555 @default.
- W2380039300 hasRelatedWork W2390407627 @default.
- W2380039300 hasRelatedWork W2460480442 @default.
- W2380039300 hasRelatedWork W2809516366 @default.
- W2380039300 hasRelatedWork W3031064969 @default.
- W2380039300 hasRelatedWork W2070891589 @default.
- W2380039300 isParatext "false" @default.
- W2380039300 isRetracted "false" @default.
- W2380039300 magId "2380039300" @default.
- W2380039300 workType "article" @default.