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- W2380043333 abstract "Objective:A liquid-liquid extraction-based reversed-phase HPLC method with fuorometric detection was validated and applied for the analysis of anandamide in human plasma.Method:After alkalization with NaHCO3,plasma were extracted by toluene and analyzed using a high performance liquid chromatography equipped with a flurescence detector.The mobile phase consisted of two eluents,solvent A(acetonitrile) and solvent B(sodium acetate,pH 6.5).The analyte was separated using gradient at a flow rate of 1 mL/min,spectrophotometric detection at 560nm with an excitation wavelength of 336nm. Results:The assay exhibited a good liner relationship with limits of detection ranging from 0.50 to 0.85ng(S/N=3).The validated quantification range was 1-30ng*ml-1.the intraday precision and interday precision were both less than 10%.Conclusion:The method is suitable to determine the concentration of Anandamide in human plasma." @default.
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- W2380043333 date "2010-01-01" @default.
- W2380043333 modified "2023-09-25" @default.
- W2380043333 title "Determination of Anandamide in human plasma using reversed - phase high performance Liquid Chromatography fluorescence detection with pre - column derivatization" @default.
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