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- W2380056144 abstract "Objective To investigate the effects of recombinant human Jagged 1 on the cytokine induced expansion of umbilical cord blood stem cells. Methods CD34+ cells from umbilical cord blood were isolated by MACS system,and divided into 4 groups cultured in following conditions:①Control group,② Jagged 1 protein,③ Cell factor,④ Jagged 1 protein and cell factor. Cell proliferation was examined by Trypan blue staining and MTT method after 6 days. Meanwhile,the expression of stem cell surface marker CD34 gene was examined by RT-PCR. Results Cell concentrations were 0.4±0.1,1.3±0.5,5.0±0.8 and 10.2±1.5(×104/mL)in the four groups,respectively. Furthermore,expression of CD34 gene was detected in all the 4 groups. Conclusion Jagged 1 enhances the expansion of umbilical cord blood stem cells in vitro. It is more effective when Jagged 1 works together with other classic growth factors." @default.
- W2380056144 created "2016-06-24" @default.
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- W2380056144 date "2010-01-01" @default.
- W2380056144 modified "2023-09-23" @default.
- W2380056144 title "Recombinant human Jagged 1 protein enhances cytokine induced expansion of umbilical cord blood stem cells" @default.
- W2380056144 hasPublicationYear "2010" @default.
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