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- W2380056170 abstract "18 fungal strains including N.coenophialum,N.lolii, N.huerfanum、N.chisosum、N.aotearoae、N.sp.and 8 varieties of grass seeds belonging to Festuca arundinacea and Lolium perenne have been studied.With amplification of IS1～IS3 and F1～R1 of genomic DNA, the primers Tub-2-F～Tub-2-R from Tubulin-2 gene and F3～R3 from NC25 gene have been designed.A PCR method to detect N.coenophialum and N.lolii was established, and also a nested-PCR method to detect N.coenophialum and N.lolii in single seed was established.These PCR detection methods are strongly special and much credible and rapid-speeded." @default.
- W2380056170 created "2016-06-24" @default.
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- W2380056170 date "2006-01-01" @default.
- W2380056170 modified "2023-09-23" @default.
- W2380056170 title "PCR Detection of N.coenophialum and N.lolii" @default.
- W2380056170 hasPublicationYear "2006" @default.
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