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- W2380069382 abstract "Objective To document the polymorphism of molecular type and serotype of Salmonella isolated from person in preventive physical examination by creating database aided with PFGE.Methods Twenty-one Salmonella stains were identified and serotyped by diagnostic serum.Then the total genomic DNA of the isolates was digested with restriction enzyme Xba I,and run pulsed-field gel electrophoresis(PFGE).The DNA fingerprints of different stains were analyzed by Bionumerics5.1 software to perform clustering.Results The 21 Salmonella strains belonged to three subgroup of O:4(B),O:9(D1),O:3,10(E1) and 8 serotype;S.Derby,accounted for 33.3%(7/21);S.Agona were 19.1%(4/21) and S.Typhimurium 14.3%(3/21);The DNA fingerprints of 18 Salmonella strains belonged to 15 PFGE pattern combinations.Similarity value of these strains was in the range of 53.9%～100%.One serotype Salmonella showed several PFGE patterns combinations.Conclusion PFGE pattern combinations of this study show polymorphism;The PFGE technology is suitable for molecular typing of Salmonella in preventive physical examination." @default.
- W2380069382 created "2016-06-24" @default.
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- W2380069382 date "2012-01-01" @default.
- W2380069382 modified "2023-09-24" @default.
- W2380069382 title "Serotyping and molecular typing Salmonella by PFGE" @default.
- W2380069382 hasPublicationYear "2012" @default.
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