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- W2380104976 abstract "To clone the cDNA fragments of the matrix metalloproteinase-9(MMP-9) gene of dairy goat and to analyze its gene sequences,the mammary gland tissues of dairy goat during lactation were collected to extract the total RNA under sterile conditions.A pair of specific primers were designed based on the conserved regions of MMP-9 gene of other known species,and the RT-PCR was performed to amplify the complete CDS sequences of MMP-9 from dairy goat.The analyses were performed including the nucleotide and deduced amino acid sequence analysis after sequencing,and the cluster analysis for the eight species.The results showed that a cDNA fragment of 2 245 bp for the MMP-9 gene was obtained,and its GenBank accession number was JQ670877,which contained a full-length CDS with a length of 2 130 bp.The nucleic acid sequence analysis revealed that the gene encoded for 709 amino acids.Compared with the nucleotide sequences of human,mouse and cattle,the similarities of the cDNA nucleotide sequences of goat MMP-9 genes were determined as 84%,80% and 96%,respectively,and the similarities of its amino acid sequence compared with that of human,mouse and cattle were determined as 79%,73% and 94%,respectively." @default.
- W2380104976 created "2016-06-24" @default.
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- W2380104976 date "2013-01-01" @default.
- W2380104976 modified "2023-09-24" @default.
- W2380104976 title "Cloning and bioinformatics analysis of cDNA for the MMP-9 gene in dairy goat" @default.
- W2380104976 hasPublicationYear "2013" @default.
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