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- W2380114448 abstract "Objective To develop the better methods of isolating and culturing mesenchymal stem cells(MSCs).Method The attachment efficiency of MSCs cultured in normal culture flask,flask rayed by Co60and Ⅳcollagen coating flask were observed,while the purity of MSCs cultured in IMDM,1640 and MesenCult medium were deceted.The expression of surface molecules were examined by flow cytometic.Results Culture flask covered by collagen type IV or rayed by Co60(6GY) was better for the adherence and growth of MSCs.The purity of MSCs cultured in mesencult serum was the highest,and the cells didn't differentiate in several passage.Flow cytometric analysis indicated that MSCs were CD34(-),CD45(-),CD71(++),CD44(++),CD90(++).Conclusion A great number of MSCs with high purity can be obtained by culturing in flask rayed by Co60(6GY) with mesencult serum free media.This study can provide a feasible empirical method for further basic research and clinical application." @default.
- W2380114448 created "2016-06-24" @default.
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- W2380114448 date "2008-01-01" @default.
- W2380114448 modified "2023-09-25" @default.
- W2380114448 title "Effect of different culturing methods on the attachment and purity of human Mesenchymal stem cells" @default.
- W2380114448 hasPublicationYear "2008" @default.
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