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- W2380159449 abstract "Aim:To investigate whether Flk1+CD31-CD34-cells isolated from adult adipose tissue have characteristics of hemangioblasts.Methods:Flk1+CD31-CD34-cells were characterized on extracellular matrix(ECM) gel or semisolid media supplemented with endothelial or hematopoietic growth factors,respectively.Immunochemistry staining and RT-PCR were performed for cell characterization.Results:After being cultured on endothelial condition,the induced cells were positive for CD31 and vWF.On ECM gel,Flk1+CD31-CD34-cells could grow into vascular structure.Additionally,Flk1+CD31-CD34-cells could differentiate into hematopoietic cells which expressed GATA-1,2,and gamma,beta globin gene,and after being replated in methylcellulose medium,they formed typical erythroid colonies.Conclusion:Adult adipose tissue-derived Flk1+CD31-CD34-cells could differentiate into endothelial and hematopoietic cells.The results suggested that these Flk1+CD31-CD34-cells bear characteristics of hemangioblast." @default.
- W2380159449 created "2016-06-24" @default.
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- W2380159449 date "2007-01-01" @default.
- W2380159449 modified "2023-09-25" @default.
- W2380159449 title "Observation of hemangioblastic characteristics of human adult adipose tissue-derived Flk1~+CD31~-CD34~-cells" @default.
- W2380159449 hasPublicationYear "2007" @default.
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