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- W2380166544 abstract "The immobilized metal affinity chromatography(IMAC) matrixes were successfully prepared with chitosan as support,formaldehyde as precrosslinking agent,glutaraldehyde as crosslinking agent,3-Chloro-1,2-epoxypropane as activated agent,ethylenediamine as chelating ligand and Zn2+ as center ions.Then it was used to separate and purify aldehyde dehydrogenase(ALDH) tagged with 6×His.The yield of ALDH was 29.0 % and the purification fold reached 9.23 times by ammonium sulfate precipitation and immobilized metal affinity chromatography.It proved that the prepared high-efficiency immobilized metal affinity chromatography matrixes could be used to rapidly separate and purify the His-tagged recombinant protein and the purification fold and recovery rate purified by chitosan Zn2+ immobilized affinity chromatography were higher than any other methods reported currently." @default.
- W2380166544 created "2016-06-24" @default.
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- W2380166544 date "2012-01-01" @default.
- W2380166544 modified "2023-09-25" @default.
- W2380166544 title "Purification of Aldehyde Dehydrogenase with Histidine-tagged by Immobilized Metal Ion Affinity Chromatography with Chitoan Used as Substrate" @default.
- W2380166544 hasPublicationYear "2012" @default.
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