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- W2380188066 abstract "Objective To establish a method of SYBR green real-time quantitative PCR for detecting mRNA levels of VEGF, and apply the method to cosmetic diseases preliminarily. Methods The recombinant plasmid of GAPDH was constructed as standard control. Standard curves of VEGF and GAPDH were established by using a series dilution of quantified plasmids and the specificity of PCR products was analysed by their melting curves. VEGF mRNA levels of normal skin, burning scar, A2058 and WM793 melanoma cell lines were measured by SYBR green real-time quantitative PCR. Results Two standard curves of VEGF and GAPDH had a linear feature and their R values were both 1. In addition, the melting curve analysis showed a single peak. The results of four groups showed that the VEGF mRNA levels were higher in burning scar tissue, A2058 cells and WM793 cells than that in normal skin tissue. Conclusion The method of detecting VEGF mRNA levels by SYBR green real-time quantitative PCR was established successfully in this study." @default.
- W2380188066 created "2016-06-24" @default.
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- W2380188066 date "2008-01-01" @default.
- W2380188066 modified "2023-09-23" @default.
- W2380188066 title "Establishment of VEGF mRNA detection by using SYBR green real-time fluorescent quantitative PCR" @default.
- W2380188066 hasPublicationYear "2008" @default.
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