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- W2380212605 abstract "Objective To explore whether quantitative standard curve may be directly extended to determine HBV DNA level in the cases of very high viral loads which was over the upper limit of detection range.Methods A total of 30 serum samples were included.The HBV DNA levels of all the samples were more than 3×107 IU/ml detected by fluorescent quantification kit purchased from Shanghai Shenyou Company.Each samples was quantitatively retested for three times.Both the serum samples and the HBV DNA extracted from serum were further measured in 10-,100-and 1 000-fold dilution,respectively.After logarithmic conversion the quantitative values were statistically analyzed.Results When the quantitative data of HBV DNA were expressed as logarithm values,the inter-class correlation coefficient was 0.356(F=2.66,P0.01) in three repeated detection of the 30 sera.The maximum differences of HBV DNA levels in the repeated detection were 0.5,0.5 to 1 and 1.01 in 11(36.7%),18(60.0%),and 1(3.3%) of the 30 samples respectively.,respectively.After the serum was diluted with 10-,100-and 1 000-fold,the intra-class correlation coefficient was 0.960(F=72.57,P0.01),with the maximum difference of 0.5(0.02 to 0.45) for all the samples except one(0.53).After the extracted DNA from serum was diluted with 10-,100-and 1 000-fold,the intra-class correlation coefficient was 0.973(F=107.66,P0.01),with the maximum difference of 0.5(0.02 to 0.44) for all the samples.Comparison of HBV DNA levels in the undiluted samples and diluted with two different methods showed that the averages of the detected results were similar(F=1.66,P0.05),and the maximum differences were less than 1 in each sample.Conclusion In the determination of HBV DNA by using domestic reagents,direct extension of the quantitative standard curve may be acceptable even if the level of HBV DNA was over the upper limit of detection.For accurate quantification,it is suggested that the serum-extracted DNA with 100-fold dilution should be used as the test materials." @default.
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- W2380212605 date "2012-01-01" @default.
- W2380212605 modified "2023-09-25" @default.
- W2380212605 title "Evaluation for quantitative methods of HBV DNA in serum with very high viral loads" @default.
- W2380212605 hasPublicationYear "2012" @default.
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