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- W2380259503 abstract "The phenomenon of negative peaks ultraviolet detection (UV) was reported and the principle was explained. In liquid chromatography, solute molecules in the mobile phase undergo many successive adsorptions and desorptions. It was proved that there was a relationship called stoichiometric displacement. The stoichiometric displacement retention behavior of samples in the column was explained using the theory. Based on the theory, the content of each component in the mixed ketones was detected. From the study, UV detector can be used to detect the sample without UV absorbance. As the result of the study, liquid chromatography separation was performed on Agilent C18 column with methanol-water (60∶40, V/V) and sulfosalicyclic acid concentration of 0.01 mmol/L as mobile phase and with UV detection (λsig=310 nm, λref=275 nm ). The method was simple and the correlation coefficients were above 0.9993. The limit of quantitative detection was 0.041 μg for methyl ketones and their relative standard deviations were between 0.5% and 0.8%." @default.
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- W2380259503 date "2010-01-01" @default.
- W2380259503 modified "2023-09-25" @default.
- W2380259503 title "Negative Peaks Ultraviolet Detection in Reversed Phase High Performance Liquid Chromatography and Its Application to Determination of Ketones" @default.
- W2380259503 hasPublicationYear "2010" @default.
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