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- W2380290438 abstract "Objective: To screen the interactive proteins of hCLP46 for exploring the role of hCLP46 function on the pathogenesis of MDS-AML. Methods: The human leukocyte cDNA library was screened with pGBKT7-hCLP46 as bait plasmid by yeast two hybrid system. Leu+, Mel+, Trp+, His+ positive yeast clones were obtained. The exogenous fragments of identified positive clones were sequenced and analyzed with Blast in NCBI. Results:There are five positive yeast clones which were obtained through screening the human leukocyte cDNA library with pGBKT7-hCLP46 as bait plasmid. Four hCLP46-iteractive candidate genes were confirmed by DNA sequencing and Blast analysis in NCBI. Conclusion:Four gene sequences were obtained with their coding proteins interacting with hCLP46 , these candidate genes possibly involve with pathogenesis of MDS-AML." @default.
- W2380290438 created "2016-06-24" @default.
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- W2380290438 date "2008-01-01" @default.
- W2380290438 modified "2023-09-24" @default.
- W2380290438 title "Screening Proteins Interacting with hCLP46 from Human Leukocyte cDNA Library Using Yeast Two-Hybrid System" @default.
- W2380290438 hasPublicationYear "2008" @default.
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