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- W2380296834 abstract "This stuffy ained to prepare monoclonal antibodies against Cor h 1 allergen from Dermatophagoides farinae and to characterize their properties.Balb/c mice were immunized with purified recombinant Cor h 1 allergen protein,and the splenocytes of the immunized mice were fused with NS-1 myeloma cells by hybridoma technique.The McAbs against Cor h 1 allergen were purified using affinity chromatography on immobilized Protein A,and identified by ELISA and Western blotting for the specificity,subtype,titers and cross-reactivity.Four hybridoma cell lines secreting McAbs against Cor h 1 allergen were obtained,which were determined as IgG1 subtype and possessed good ascites titers.The four McAbs against Cor h 1 allergen all recognized recombinant Cor h 1 allergen,and the three of them also recognized native extract from Corylus heterophylla.In our study,four monoclonal antibodies against Cor h 1 allergen have prepared successfully,which will facilitate the establishment of detection method for Cor h 1 allergen." @default.
- W2380296834 created "2016-06-24" @default.
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- W2380296834 date "2011-01-01" @default.
- W2380296834 modified "2023-09-27" @default.
- W2380296834 title "Preparation and identification of monoclonal antibodies against Cor h1 allergen" @default.
- W2380296834 hasPublicationYear "2011" @default.
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