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- W2380304042 abstract "Objective To investigate splenocyte proliferation in mice immunized with transgenic alfalfa(Medicago sativa) containing the Eg95-EgA31 fusion gene of Echinococcus granulosus(Eg) and challenged with Eg protoscoleces.Methods Leaf protein was extracted from transgenic alfalfa by heat coagulation and prepared at a concentration of 20 μg/μl.BALB/c mice were immunized intranasally or orally with leaf protein once every 3 days for 2 months.At the same time,leaf protein transfected with a pBI-121 blank vector and normal leaf protein without foreign antigens served as controls.The mice were challenged intraperitoneally with Eg protoscoleces(50 protoscoleces per mouse) on week 8 after the last vaccination and sacrificed on week 24 post-infection to count the rate of reduced hydatid cyst weight.Splenocytes were separated for culturing in vitro with EgAg or ConA stimulus.Splenocyte proliferation was determined by MTT method.Results The hydatid cyst weight in the oral immunization group was reduced 64.1% compared to that in the control group.Splenocyte proliferation in both immunized groups increased significantly in comparison to that in the control group,and the level of splenocyte proliferation in the oral group was obviously higher than that in the intranasal group.Conclusion The proliferation of splenocytes can be induced in mice by immunization with transgenic alfalfa.Oral immunization may be an effective route of immunization." @default.
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- W2380304042 date "2010-01-01" @default.
- W2380304042 modified "2023-09-26" @default.
- W2380304042 title "Study on proliferation of splenocytes in mice resulting from immunization with transgenic Alfalfa (Medicago sativa) containing the Eg95-Ega31 fusion gene of Echinococcus granulosus." @default.
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