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- W2380308713 abstract "OBJECTIVE To analyze the ITS sequences of Chinese Aconitum species,including A.carmichaeli,A.kusnezoffii and those which are medicinally possibly used as their substitutes,and to evaluate the utility of ITS sequences in the molecular authentication of the herbal medicinal aconites.METHODS ITS of 20 taxa of Aconitum were amplified using PCR systems and directly sequenced by an ABI 377 automated sequencer.RESULTS The sequencer analysis showed that the ITS 1 ranged from 248 to 252 bp,and the ITS 2 from 214-217 bp,and the size of the 5.8S coding region was 164 bp for all the species studied.Chuanwu,Caowu and their adulterant species were shown to be different from each other in their characters of ITS sequences,base frequencies and the pairwise genetic distances.CONCLUSION ITS region could be adopted as a molecular marker for differentiating medicinal Aconitum species." @default.
- W2380308713 created "2016-06-24" @default.
- W2380308713 creator A5083848843 @default.
- W2380308713 date "2008-01-01" @default.
- W2380308713 modified "2023-09-23" @default.
- W2380308713 title "Study on ITS Sequences of Herbal Medicine of Chuanwu and Caowu" @default.
- W2380308713 hasPublicationYear "2008" @default.
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