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- W2380308950 abstract "Objective:To evaluate the risk of usual disinfections with ultraviolet radiation in high-level biosafety laboratory to offer the experimental data for the establishment of a standard operative technique.Methods:A model bacteria,deriving from E.coli strain DH5α,was constructed,into which the plasmid pUC19 was transformed.This recombinant was used to simulate the circumstances,which were probably encountered by the operations in high-level biosafety laboratories.Thus the survival of bacterial after exposure to ultraviolet radiation was evaluated.Results:For a small quantity of bacteria,the ultraviolet radiation could kill all the bacteria in a biosafety cabinet in 10 min,and in the laboratory in 30 min within 2 meters far from the UV light.While for a large quantity of bacteria,the ultraviolet radiation worked unsatisfactorily.In the biosafety cabinet,ultraviolet radiation could kill all the bacteria dropped on the surface of biosafety cabinet,exposure suit or gloves in 10 min.Conclusion:For the pollution of large quantity of bacteria,simple ultraviolet radiation was a disinfection method underlying danger,thus it is not recommended to regard the ultraviolet radiation as the only method for high-level biosafety laboratories." @default.
- W2380308950 created "2016-06-24" @default.
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- W2380308950 date "2009-01-01" @default.
- W2380308950 modified "2023-09-24" @default.
- W2380308950 title "Establishment of a model to evaluate efficiency of disinfection with ultraviolet radiation in high-level biosafety laboratory" @default.
- W2380308950 hasPublicationYear "2009" @default.
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