Matches in SemOpenAlex for { <https://semopenalex.org/work/W2380330168> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2380330168 abstract "HER 2/neu receptor ligand domain 2 (RLD2) cDNA was amplified by PCR, cloned into the prokaryotic expression vector containing thioredoxin A (TrxA) gene and expressed in soluble form as TrxA RLD2 fusion protein. TrxA and RLD2 was co expressed by inserting a tandem sequence of stop and start codon into the recombinant plasmid. Western blotting showed that the expressed protein could react with the specific antibody against proto oncoprotein HER 2/neu. With the application of DEAE Sepharose FF and cobalt based affinity resin, the purity of RLD2 obtained was over 90%. The purified protein was further subject to mass spectroscopy molecular weight monitoring, and the molecular weight of RLD2 protein was identical to that expected. By using TrxA expression system, the soluble expression of RLD2 protein was obtained with high efficiency." @default.
- W2380330168 created "2016-06-24" @default.
- W2380330168 creator A5043119710 @default.
- W2380330168 date "2004-01-01" @default.
- W2380330168 modified "2023-09-24" @default.
- W2380330168 title "Soluble Expression and Purification of HER-2/neu Extracellular Receptor Ligand Domain 2" @default.
- W2380330168 hasPublicationYear "2004" @default.
- W2380330168 type Work @default.
- W2380330168 sameAs 2380330168 @default.
- W2380330168 citedByCount "0" @default.
- W2380330168 crossrefType "journal-article" @default.
- W2380330168 hasAuthorship W2380330168A5043119710 @default.
- W2380330168 hasConcept C104317684 @default.
- W2380330168 hasConcept C123894998 @default.
- W2380330168 hasConcept C153911025 @default.
- W2380330168 hasConcept C179247698 @default.
- W2380330168 hasConcept C181199279 @default.
- W2380330168 hasConcept C185592680 @default.
- W2380330168 hasConcept C187882448 @default.
- W2380330168 hasConcept C22744801 @default.
- W2380330168 hasConcept C2779881004 @default.
- W2380330168 hasConcept C28406088 @default.
- W2380330168 hasConcept C33161422 @default.
- W2380330168 hasConcept C3623737 @default.
- W2380330168 hasConcept C40767141 @default.
- W2380330168 hasConcept C55493867 @default.
- W2380330168 hasConcept C58121356 @default.
- W2380330168 hasConcept C85265743 @default.
- W2380330168 hasConcept C86803240 @default.
- W2380330168 hasConceptScore W2380330168C104317684 @default.
- W2380330168 hasConceptScore W2380330168C123894998 @default.
- W2380330168 hasConceptScore W2380330168C153911025 @default.
- W2380330168 hasConceptScore W2380330168C179247698 @default.
- W2380330168 hasConceptScore W2380330168C181199279 @default.
- W2380330168 hasConceptScore W2380330168C185592680 @default.
- W2380330168 hasConceptScore W2380330168C187882448 @default.
- W2380330168 hasConceptScore W2380330168C22744801 @default.
- W2380330168 hasConceptScore W2380330168C2779881004 @default.
- W2380330168 hasConceptScore W2380330168C28406088 @default.
- W2380330168 hasConceptScore W2380330168C33161422 @default.
- W2380330168 hasConceptScore W2380330168C3623737 @default.
- W2380330168 hasConceptScore W2380330168C40767141 @default.
- W2380330168 hasConceptScore W2380330168C55493867 @default.
- W2380330168 hasConceptScore W2380330168C58121356 @default.
- W2380330168 hasConceptScore W2380330168C85265743 @default.
- W2380330168 hasConceptScore W2380330168C86803240 @default.
- W2380330168 hasLocation W23803301681 @default.
- W2380330168 hasOpenAccess W2380330168 @default.
- W2380330168 hasPrimaryLocation W23803301681 @default.
- W2380330168 hasRelatedWork W1981241848 @default.
- W2380330168 hasRelatedWork W1989765008 @default.
- W2380330168 hasRelatedWork W2020933791 @default.
- W2380330168 hasRelatedWork W2076183276 @default.
- W2380330168 hasRelatedWork W2092918492 @default.
- W2380330168 hasRelatedWork W2348114544 @default.
- W2380330168 hasRelatedWork W2349423150 @default.
- W2380330168 hasRelatedWork W2352931556 @default.
- W2380330168 hasRelatedWork W2354736340 @default.
- W2380330168 hasRelatedWork W2356623277 @default.
- W2380330168 hasRelatedWork W2359958847 @default.
- W2380330168 hasRelatedWork W2367347068 @default.
- W2380330168 hasRelatedWork W2369699306 @default.
- W2380330168 hasRelatedWork W2370606119 @default.
- W2380330168 hasRelatedWork W2371423532 @default.
- W2380330168 hasRelatedWork W2376615012 @default.
- W2380330168 hasRelatedWork W2387256401 @default.
- W2380330168 hasRelatedWork W2393038909 @default.
- W2380330168 hasRelatedWork W2394333668 @default.
- W2380330168 hasRelatedWork W3141588926 @default.
- W2380330168 isParatext "false" @default.
- W2380330168 isRetracted "false" @default.
- W2380330168 magId "2380330168" @default.
- W2380330168 workType "article" @default.