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- W2380341391 abstract "Porcine circovirus type 2(PCV2) is an important causative agent of postweaning multisystemic syndrome in pigs,and both replicase proteins(Rep' and Rep) encoded by PCV2-ORF1 are essential for the viral replication.The monoclonal antibody(MAb) to the Rep' protein was prepared by means of lymphocyte hybridoma technique.The MAb isotype was belong to be IgG1/κ.The antibody titer of ascites was 1∶819,200.Western blot and IPMA analysis showed that the MAb was able to react with both PCV2-rRep' and PCV2-rRep proteins expressed by baculovirus system and PCV2 infected cells.Furthermore,the Rep' protein antigenic epitope was mapped by the synthesized peptides to scan with the MAb.The epitope core sequence was identified as 61FANFVKKQTFNKV73,and it located at the nuclear localization signal region of the N terminal of Rep' protein.This MAb and its corresponding epitope mapping provide a basis for further study on the viral molecular biology and diagnosis technique." @default.
- W2380341391 created "2016-06-24" @default.
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- W2380341391 date "2013-01-01" @default.
- W2380341391 modified "2023-09-23" @default.
- W2380341391 title "Preparation of the monoclonal antibody against the Rep' protein of porcine circovirus type 2 and its antigenic epitope identification" @default.
- W2380341391 hasPublicationYear "2013" @default.
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