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- W2380344511 abstract "Objective:To establish a HPLC-DAD method for the determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum L.Methods:Kromasil C18 column(4.6 mm×250 mm,5 μm)was adopted.The mobile phase consisted of methanol-0.5% phosphoric acid aqueous solution(10∶90)at a flow rate of 0.8 mL·min-1,and the column temperature was set at 30 ℃.The detection wavelength was 210 nm.Results:Oleanolic acid showed good relationship at the range of 0.228-2.850 μg(r=0.9998)and the recovery was 97.4% with 1.5%(n=6)of RSD.Ursolic acid showed good relationship at the range of 0.544-6.800 μg(r=0.9998)and the recovery was 96.9% with 1.2%(n=6)of RSD.Conclusion:The proposed method is accurate,simple,and promises to be applicable for the determination of oleanolic acid and ursolic acid in the different parts of P.perfoliatum L." @default.
- W2380344511 created "2016-06-24" @default.
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- W2380344511 date "2011-01-01" @default.
- W2380344511 modified "2023-09-23" @default.
- W2380344511 title "HPLC-DAD determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum" @default.
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