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- W2380349747 abstract "In order to know function of cysDN and its effect on plant nodulation,a random insertion mutant library of Sinorhizobium fredii 15142 was constructed by three parental hybridization with the plasmid pTnMod-RKm' as transposon vector.A mutant strain which could not metabolize sulfate while could use cysteine as sulfur source,was selected by MM medium with different sulfur sources from the library.Molecular cloning and sequence analysis showed that this operon had 92% and 96% similarity with the cysDN gene sequence of Sinorhizobium sp.BR816 at nucleotide and amino acid level,respectively.With the suicide plasmid pK18mob,the polar mutant cysDF/15142,cysNF/15142 and non-polar mutant cysDR/15142,cysNR/15142 inactivated in cysD and cysN were constructed through homologous recombination.A recombinant plasmid pLAFRJ+cysDN was constructed by using plasmid pLAFRJ ligated with cysDN,and transferred into above mutants to obtain complement strains.The results of growth tests found that the mutants had no growth on the MM medium with sulfate as sulfur source but the complement strains could grow normally,indicating that cysDN operon was related to sulfate assimilation.The plant tests indicated that the mutant strains delayed 1~2 d to nodulate than the wild strain,and nitrogenase activity and nodulation efficiency in mutants were slightly lower than those of the original strain,but there were no differences in the number and weight of nodules and the dry weight of plant." @default.
- W2380349747 created "2016-06-24" @default.
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- W2380349747 date "2011-01-01" @default.
- W2380349747 modified "2023-09-26" @default.
- W2380349747 title "Cloning and Related Function of cysDN in Sinorhizobium fredii 15142" @default.
- W2380349747 hasPublicationYear "2011" @default.
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