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- W2380367427 abstract "Objective:To establish a stable and repeatable HPLC reference fingerprint chromatogram standard and a colorimetric assay method for qualitative and quantitative evaluation of pueraia isoflavones in Pueraria thomsonii and Pueraria lobata.Methods:HPLC separation was performed on a C18 column with methanol-0.1% formic acid solution as mobile phase in gradient elution mode.The reference fingerprint chromatogram was determined as one of the most typical chromatograms that represented the property of pueraia isoflavones in Pueraria thomsonii and Pueraria lobata from both constitutes and quantity view of points,established by statistical and similarity analysis.The colorimetric assay method was established with Folin-phenol as the coloring reagent,puerarin as the reference standard,coloring reaction in 70 ℃ water bath for 15 min,and absorbance measurement at 750 nm.The total pueraria isoflavones were determined by calibration curve method with the sample blank as reference.Results:The HPLC method was specific and having good resolution for the pueraia isoflavones.The fingerprint chromatograms of Pueraria thomsonii and Pueraria lobata showed 20 and 11 characteristic peaks,respectively.Calibration curve of the colorimetric method was linear in the range from 12 to 40 mg·L-1 with r=0.999 5.The average recovery was 101.1%(n=6).Conclusion:The pueraia isoflavones fingerprint chromatograms of Pueraria thomsonii and Pueraria lobata can be quantitatively and qualitatively evaluated by comparison with the established reference fingerprint chromatograms by using Cosine q and relative Euclid distance methods,respectively.The method is of good repeatability and ruggedness,and more comprehensive and practical.The colorimetric assay method for the total pueraia isoflavonoids is sensitive and accurate." @default.
- W2380367427 created "2016-06-24" @default.
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- W2380367427 date "2008-01-01" @default.
- W2380367427 modified "2023-09-28" @default.
- W2380367427 title "Determination of pueraia isoflavones in Pueraria thomsonii and Pueraria lobata by HPLC reference fingerprint chromatogram and Folin-phenol reagent colorimetry" @default.
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