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- W2380377711 abstract "A polymerase chain reaction (PCR) assay test was developed for the detection of mecA gene in methicillin-resistant Staphylococcus aureus (MRSA). The sensitivities in four of DNA extraction methods from Staphylococcus aureus were sequentially lysis cell by ultrasonics (5×105 CFU/ml), lysozymesurfactant (5×106 CFU/ml), surfactant (1×107 CFU/ml) and directly boiling (1×108 CFU/ml). There was a simplicity in the DNA extraction by directly boiling. A pair of primers that corresponded to nucleotides 181 to 200 as the sense strand with sequence 5'-GAAATGACTGAACGTCCGAT and 311 to 330 as the antisense strand with 5'-GCGATCAATGTTACCGTAGT were selected for amplifing a 150 bp-long segment of the mecA gene. The better specificity was shown by the results of 5 species strains tested. There was an excellant relationship between the presence of the mecA gene and the level of bacteria to oxacillin MICs. The fact that the mecA gene were detected from all of 22 strains with the MIC≥4μg/ml to oxacillin suggested the reliability for routinely determing MRSA by the MIC levels to oxacillin. There was most importance in detecting cryptically methicillin-resistant strains by PCR technique." @default.
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- W2380377711 date "1995-01-01" @default.
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- W2380377711 title "Detection of Methicillin-resistant Staphylococcus Aureus mecA gene by polymerase chain reaction" @default.
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