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- W2380398128 abstract "AIM To investigate the protective effect of zincon the microwave induced lipid peroxide damage to in vitro cultured pig retinal ganglion cells and the cells' tolerance to microwave. METHODS Pig retinal ganglion cells were cultured in vitro and divided into 3 groups of different radiation intensities (control group, 30 mW·cm -2 group, 60 mW·cm -2 group) and 2 more radiated groups with different dosages of zinc added. The activity of intracellular superoxide dismutase (SOD) and the content of malondia ldehyde (MDA) of each group were assayed after they had been radiated for 1 h in a shielded room by 2450 MHz continuous wave physiotherapy machine. 0 and 72 h after radiation, the morphological variation of cells was observed by optical microscopy and the activity of intracellular SOD and the MDA content were determined.RESULTS After radiation, some cells congregated and their axons disappeared. The content of MDA increased significantly compared with that of the control group and whereas activity of SOD decreased. No obvious change was discovered in the zinc added groups by microscopy. The content of MDA recovered and activity of SOD in the zinc added groups increased compared with that of the radiated groups without zinc added. With cells cultured for 72 h after radiation, the content of MDA of the 30 mW·cm -2 group decreased and there was no significant difference between the MDA content of the 60 mW·cm -2 group and that of the control. MDA decreased whereas SOD increased in zinc added groups after the 72 h culture, compared with that in the radiation groups. CONCLUSION The results of this study indicate that microwave can induce lipid peroxide damage to in vitro cultured retinal ganglion cells and that Zinc can improve antioxidative capacity of cells, and to some extent can remedy microwave induced damage of lipid peroxidation to retinal ganglion cells. Besides, the effect of zinc is related to its action time." @default.
- W2380398128 created "2016-06-24" @default.
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- W2380398128 date "2000-01-01" @default.
- W2380398128 modified "2023-09-28" @default.
- W2380398128 title "Effect of Zinc on lipid peroxidation induced by microwave" @default.
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