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- W2380427337 abstract "To study the isolated culture system of porcine fetal fibroblasts and ear skin fibroblasts for selecting the best culture method according to the samples from different tissues,porcine fetal and ear skin were used as experimental material,the porcine fetal fibroblasts and ear skin fibroblasts were isolated and cultured by four different methods including the methods for trypsin hot digestion,trypsin digestion of hot and cold combination,trypsin digestion at room temperature and tissue culture.The effects of the four methods were compared to find a better one.The results showed that the survival rate of porcine fetal fibroblasts isolated by the method for trypsin digestion at room temperature,was significantly higher than those by the methods for trypsin hot digestion and trypsin digestion of hot and cold combination(P0.05).The method for trypsin digestion at room temperature was simple to operate,and the effect of cell adhesion was better.In primary cultured ear skin fibroblasts,a small number of cells were isolated by the methods for trypsin hot digestion and trypsin digestion at room temperature.The method for tissue culture needed a small number of tissues,and was easier to operate than the method for trypsin digestion of hot and cold combination.The cultured cells were in typical fibroblast morphology,the growth curve was sigmoidal,and the cells grew well after freezing and thawing.The results indicate that the method for trypsin digestion at room temperature is a good culture method is for porcine fetal fibroblast,and the methods for trypsin hot digestion and the trypsin digestion at room temperature are the ideal culture methods for primary cultured porcine ear skin fibroblasts." @default.
- W2380427337 created "2016-06-24" @default.
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- W2380427337 date "2013-01-01" @default.
- W2380427337 modified "2023-09-25" @default.
- W2380427337 title "A study of culture methods for porcine fetal fibroblasts and ear skin fibroblasts" @default.
- W2380427337 hasPublicationYear "2013" @default.
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