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- W2380446445 abstract "Four different DNA extraction methods for activated sludge were evaluated. They are enzymatic lysis, chemical lysis, bead mill homogenization and freeze-thawing. The effects of them were compared based on the yield, purity, molecular size distribution of the recovered DNA, and existence of PCR-inhibitor. The bias of the four methods was evaluated according to the ITS PCR products length polymorphism. The results show that the chemical lysis is most effective and has the most total DNA yield and large size fragments, the impurities in the products by chemical lysis do not inhibit the PCR, and the bias of this method is the least." @default.
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- W2380446445 date "2004-01-01" @default.
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- W2380446445 title "Evaluation of Different DNA Extraction Methods for Activated Sludge" @default.
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