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- W2380452757 abstract "Human membrane cofactor protein(MCP,CD46) is a cell surface glycoprotein acting as a cofactor for the factor Ⅰ-mediated inactivation of C3b and C4b.The purpose of this study is to obtain the cDNA sequence encoding human MCP for further study.cDNA fragment was amplified with PCR using adult liver cDNA library as template.The fragment was cloned into pUC19 plasmid,and further sequenced using Dye Terminator Cycle Sequencing Kit with AmpliTaq DNA Polymerase by ABI PRISMTM 377 DNA Sequencer.The cDNA fragment has 1 110 bp with open reading region,which encoded the signal peptide,four extracellular short consensus repeat domains(SCRs),a Ser/Thr(STC)-rich region,a transmembrane region and a cytoplasmic tail(CYT2).Its sequence was identical to the published sequence encoding ST C CYT2 isoform of human MCP." @default.
- W2380452757 created "2016-06-24" @default.
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- W2380452757 date "2002-01-01" @default.
- W2380452757 modified "2023-09-25" @default.
- W2380452757 title "Cloning and Sequencing of Human Complement Membrane Cofactor Protein" @default.
- W2380452757 hasPublicationYear "2002" @default.
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