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- W2380453842 abstract "Objective To construct two different luciferase reporter plasmid pGL4.26-PEPCK-Luc2,and to evaluate the expression efficiency of the phosphoenolpyruvate carboxykinase(PEPCK) gene promoters in several cell lines in vitro.Methods Two PEPCK promoter fragments 1323 bp and 560 bp were obtained from rat genomic DNA by PCR,and the fragments were inserted in pGL4.26-Luc2 luciferase reporter plasmid.Then the recombinant plasmid was transfected into hepatoma and adipocyte cell lines to compare the PEPCK driven luciferase expression efficiency.Results Restriction enzyme digestion and nucleotide sequencing conformed that the coupling site of the recombinant was correct without base mutation and deletion.The luciferase was expressed in the cell lines transfected with different plasmids pGL4.26-PEPCK-Luc2.Conclusion The expression efficiency of the two different PEPCK promoters in various cell lines has no significant difference." @default.
- W2380453842 created "2016-06-24" @default.
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- W2380453842 date "2010-01-01" @default.
- W2380453842 modified "2023-09-26" @default.
- W2380453842 title "Comparison of the Expression Efficiency of Two Different Recombinant Firefly Luciferase Reporter Plasmids pGL4-PEPCK-Luc2" @default.
- W2380453842 hasPublicationYear "2010" @default.
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