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- W2380456655 abstract "The ABC-ELISA assay was used to detect the mite-specific IgE in sera of 76 asthmarics and 30 healthy individuals This method of testing was performed on the proper conditions:when the optic density (OD) value of positive sera was 1.0.,the best concentration of coating antigen,serum dilutions and concentration of enzyme-conjugates were pre-tested before use and verified by specific inhibition test.The results were compared with those of skin prick test (SPT).It was found the best concentration of coating antigen was 10μg/ml.; the proper dilution of serum was 1∶40 and the concentration of avidin-HRP was 1∶600.Both the extract of Dermatophagoides farine and the mouse anti-human IgE antibody could suppress the positive reactions and the corrected OD values of the asthmatics and healthy individuals were 2.41±1.13 and 1.25±0.56 with significant differences (χ2=5.36,P0.01).The coincidence rate between the results of the SPT with P.farine extract and those with ABC-ELISA was 93.4% (99/106) It concludes that ABC-ELISA is a method of choice for the detection of the mite-specific IgE in sera of asthmatics,because of its short time of performance and good antigenic specificity." @default.
- W2380456655 created "2016-06-24" @default.
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- W2380456655 date "2004-01-01" @default.
- W2380456655 modified "2023-09-24" @default.
- W2380456655 title "Detection of mite-specific IgE in sera of asthmatics by ABC-ELISA" @default.
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